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KMID : 1100220040030020093
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Dementia and Neurocognitive Disorders
2004 Volume.3 No. 2 p.93 ~ p.98
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The effects of Ginkgo biloba extract (EGb 761) on Ethanol-Induced Cytotoxicity in PC12 Cells
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Huh En-Hee
Lee Jong-Keol
Han Seol-Heui
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Abstract
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Background and Objectives: The processes underlying ethanol (EtOH)-induced neurotoxicity appear to be complex, with evidence for both apoptosis and necrosis in vitro. Oxidative stress has been implicated in the cytotoxic actions of EtOH. We investigated in rat pheochromocytoma (PC12) cells to see if EtOH-induced cytotoxicity is associated with oxidative stress and the standardized extract of Ginkgo biloba extract (EGb 761) is able to attenuate EtOH-induced neurotoxicity.
Methods: We assessed oxidative stress induced by incubating PC12 cells with EtOH for 30 min by using the 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) assay quantitatively. To investigate the neuroprotection effect of EGb 761, cells were pretreated with this drug and the degree of oxidative stress or cell survival was assessed.
Results: PC12 cell cultures exhibited a loss of cells and increase in intracellular reactive oxygen species when exposed to EtOH. Loss of cell numbers was EtOH concentrationdependent. EtOH-induced neurotoxicity correlated with oxidative stress as reflected by DCFH-DA fluorescence. Addition of EGb 761 prior to EtOH treatment attenuates the EtOH-induced free radical production and neuronal death.
Conclusions: The present investigation provides evidence that oxidative stress is involved in the pathogenesis of EtOH-induced neurotoxicity in PC12 cells. Further, the neuroprotective effect of EGb 761 on EtOH toxicity correlates with the suppression of intracellular oxidative stress. These results suggest that EGb 761 is beneficial for neuronal cell survival in response to oxidative stress, such as EtOH-induced neurotoxicity.
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KEYWORD
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Ethanol, Reactive oxygen species (ROS), EGb 761, PC12 cells
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